Abstract
This work reports Tobacco mosaic virus (TMV)-based assembly of glucose oxidase (GOx) electrodes for the development of advanced enzymatic biofuel cells (EBCs). Cysteine-modified TMV (TMV1cys), self-assembled on Au electrodes, has served as a template for augmenting the GOx immobilization density via a robust chemical conjugation route. Enhanced enzymatic activity of the TMV1cys/GOx electrodes have been confirmed via colorimetric assay, and the electrochemical transduction of the catalytic reactions via mediated electron transfer resulted in a ~25-fold increase in electrochemical current compared to previous work using a similar strategy. This enhancement is attributed to the on-chip bioconjugation strategy presented in this work, leveraging the robust and high density self-assembly of TMV1cys on Au surface. The performance of an EBC consisting of TMV-assembled enzyme electrodes is reported for the first time, generating a maximum power density of 860 nW/cm2.
Published Version
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