Abstract
BackgroundThe purpose of this study was to evaluate the virulence profiles of Pseudomonas aeruginosa clinical strains recently isolated from patients hospitalized for chronic leg ulcers in the Dermatology Department of Central Military Emergency University Hospital “Carol Davila”, Bucharest, Romania.MethodsThe phenotypic screening evaluated eight soluble virulence factors (haemolysins, lecithinase, lipase, caseinase, gelatinase, amylase, DNase, aesculin hydrolysis), as well as adherence ability (Cravioto adapted method) and invasion capacity on HeLa cells (gentamicin protection assay). Seven virulence genes encoding for protease IV, 3 exoenzymes (exoS, exoT, exoU), two phospholipases plcH- haemolytic phospholipase C and plcN- non-haemolytic phospholipase C) and alginate were investigated by PCR.ResultsThe pore forming toxins and enzymes were expressed in variable proportions, the majority of the tested strains producing beta haemolysin (92.3 %), lipase (76.9 %) and lecithinase (61.5 %). The most frequent virulence genes detected in the analyzed strains were the ExoT (100 %) and AlgD (92.3 %) genes, genes codifying for phospholipases (84.6 % each of them) and for protease IV (61.5 %).ConclusionsThis study reveals that correlating virulence profiles and infection clinical outcome is very useful for setting up efficient preventive and therapeutic procedures for hospitalized patients with chronic leg ulcers and positive P. aeruginosa cultures.
Highlights
IntroductionPseudomonas aeruginosa, one of the most common bacteria isolated from chronic wounds [1], is an Besides intrinsic and acquired resistance to many antibiotics, P. aeruginosa expresses many extracellular virulence factors (exotoxins S and T with ADP-ribosylating activity which cause disruption of the host cell cytoskeleton, Georgescu et al BMC Infectious Diseases 2016, 16(Suppl 1): cytotoxin ExoU with phospholipase activity and cytotoxin Exo Y with an adenylate cyclase activity, phospholipases, protease IV and elastase) and surface associated components, as alginate
The purpose of this study was to evaluate the virulence profiles of Pseudomonas aeruginosa clinical strains recently isolated from patients hospitalized for chronic leg ulcers in the Dermatology Department of Central Military Emergency University Hospital “Carol Davila”, Bucharest, Romania
Isolation and identification of bacterial strains This study was conducted on a total of 12 P. aeruginosa clinical strains isolated during July 2013 - June 2015, from 130 patients hospitalized for chronic leg in the Department of Dermatology of the Central Military Emergency University Hospital “Carol Davila”, Bucharest, Romania
Summary
Pseudomonas aeruginosa, one of the most common bacteria isolated from chronic wounds [1], is an Besides intrinsic and acquired resistance to many antibiotics, P. aeruginosa expresses many extracellular virulence factors (exotoxins S and T with ADP-ribosylating activity which cause disruption of the host cell cytoskeleton, Georgescu et al BMC Infectious Diseases 2016, 16(Suppl 1): cytotoxin ExoU with phospholipase activity and cytotoxin Exo Y with an adenylate cyclase activity, phospholipases, protease IV and elastase) and surface associated components, as alginate. In a longitudinal survey regarding patients with non-healing venous ulcers, P. aeruginosa was isolated ≥ 3 years from the same patients, and a longer wound duration compared to all analyzed patients was revealed [10]
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