Abstract
.This study describes the natural history of dengue virus (DENV) infection in rhesus monkeys exposed to the bites of DENV-infected Aedes aegypti mosquitoes. Dengue virus–infected mosquitoes were generated by either intrathoracic inoculation or by oral feeding on viremic blood meals. Each of the six rhesus monkeys that were fed upon by intrathoracically infected mosquitoes developed non-structural protein 1 (NS1) antigenemia and an IgM response; viremia was detected in 4/6 individuals. No virological or immunological evidence of DENV infection was detected in the three monkeys exposed to mosquitoes that had been orally infected with DENV. These results demonstrate the utility of mosquito-borne challenge of rhesus monkeys with DENV.
Highlights
Dengue is the most widespread arthropod-borne viral disease in the world
As the aim of this work was to explore the reliability and features of dengue virus (DENV) infection delivered by direct mosquito bites to Nonhuman primates (NHPs), we provide a descriptive summary of the kinetics of RNAemia, non-structural protein 1 (NS1) antigenemia, and IgM/IgG antibodies
NHPs fed upon by mosquitoes that had been orally infected with DENV-3 did not result in infection
Summary
The causative agent, dengue virus (DENV), is transmitted in a human–mosquito–human transmission cycle, primarily involving Aedes aegypti mosquitoes. Intervention strategies aimed at inhibiting the transmission cycle of DENV between humans and mosquitoes are at various stages of testing. One of these strategies involves the use of Wolbachia to reduce the vector competence of Ae. aegypti populations for medically important arboviruses.[1,2,3] Conventionally, researchers assess mosquito vector competence in an in vitro transmission (IVT) assay. The IVT assay involves the restraint and subsequent collection of saliva from individual mosquitoes[4] which is tested for the presence of virus, using either direct or indirect detection methods.[5,6,7,8,9,10]
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