Abstract

Two new isolates of bovine lentivirus, also known as bovine immunodeficiency-like virus (BIV), were obtained from a seropositive cattle herd in Florida. This is the first report of new isolates of BIV since the original BIV strain, R29, was isolated in 1969. The two new BIV isolates were derived from blood buffy coat cells cocultivated in vitro with fetal bovine lung (FBL) cell cultures. The new isolates differed in vitro from the original R29 isolate in replication and syncytial formation in FBL cells. Both new isolates were confirmed as BIV by immunofluorescence, Western blot, and polymerase chain reaction (PGR). Sequence analyses of the PGR po/gene product showed a 92.6% and 93.6% homology to the published nucleotide sequence of BIV R291 Virology Cattle Research, National Animal Disease Center, ARS/USDA, Ames, Iowa 50010 2 Department of Microbiology, Immunology and Preventative Medicine, Iowa State University, Ames, Iowa 50010 3 Department of Microbiology, University of Kansas, Lawrence, Kansas 66045 4 Corresponding Author

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