Abstract
Bovine ISG15 (bISG15) is an interferon inducible ubiquitin-like protein that is responsible for the establishment of early pregnancy in ruminant, understanding the properties of bISG15 capable of being inducible in fetal bovine lung (FBL) cells upon infection of bovine immunodeficiency virus (BIV) is of significant importance. In this study, we investigated the expression of bISG15 in poly I:C treated FBL cells. The increased expression of bISG15 was observed, and the inhibition of BIV replication was also detected in FBL cells. Elimination of bISG15 expression by small interfering RNA reversed the bISG15 mediated inhibition of BIV replication. These findings demonstrate that bISG15 plays an important role in inhibition of the BIV replication in FBL cells. Furthermore, real-time PCR and western blot assay revealed that bISG15's expression can also be induced in BIV infected FBL cells. Taken together, bISG15 is an antiviral and inducible protein in BIV infected FBL cells.
Highlights
Bovine ISG15 is an interferon inducible ubiquitin-like protein that is responsible for the establishment of early pregnancy in ruminant, understanding the properties of bISG15 capable of being inducible in fetal bovine lung (FBL) cells upon infection of bovine immunodeficiency virus (BIV) is of significant importance
FBL cells were chosen to be as a target type of cells here, since our lab found that FBL cells could be infected by viruses which are capable of infecting cattle, such as bovine viral diarrhea virus (BVDV), bovine foamy virus (BFV), and bovine immunodeficiency virus (BIV)
We further try to address whether the replication of BIV can be repressed in FBL cells treated with poly I:C and to address extensively whether the expression of bISG15 is changed in FBL cells infected with BIV
Summary
Bovine ISG15 (bISG15) is an interferon inducible ubiquitin-like protein that is responsible for the establishment of early pregnancy in ruminant, understanding the properties of bISG15 capable of being inducible in fetal bovine lung (FBL) cells upon infection of bovine immunodeficiency virus (BIV) is of significant importance. We have found that expression of bISG15 can be induced in fetal bovine lung (FBL) cells after treated with poly I:C or lipopolysaccaride (LPS) [15]. We previously demonstrated that bISG15 can be induced robustly in poly I:C treated FBL cells [15]. We further try to address whether the replication of BIV can be repressed in FBL cells treated with poly I:C and to address extensively whether the expression of bISG15 is changed in FBL cells infected with BIV.
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