Vibrio cholerae was found in cultured bullfrog.

Yibin Yang,Yi Song,Yongtao Liu,Xiaohui Ai,Xia Zhu,Yuhua Chen,Haixin Zhang

doi:10.1017/s0950268822000164

Abstract

Bullfrog is one of the most important economic aquatic animals in China that is widely cultured in southern China and is a key breed recommended as an industry of poverty alleviation in China. During recent years, a fatal bacterial disease has often been found in cultured bullfrogs. The clinical manifestations of the diseased bullfrogs were severe intestinal inflammation and an anal prolapse. A bacterial pathogen was isolated from the diseased bullfrog intestines. The bacterium was identified as Vibrio cholerae using morphological, biochemical and 16S rRNA phylogenetic analysis. In this study, V. cholerae was isolated and identified in diseased bullfrogs for the first time, providing a basis for the diagnosis and control of the disease. Therefore, attention should be paid to the modes of transmission of V. cholerae from bullfrog and formulate reasonable safety measures.

Highlights

Bullfrog belongs to the Ranae family and Rana genus in the order Anura and class Amphibia of the phylum Chordata
The diseased bullfrogs showed no obvious symptoms on the surface of their body
Along with large developments in the bullfrog breeding industry, diseases are more frequently observed during the breeding process

Summary

Introduction

Bullfrog belongs to the Ranae family and Rana genus in the order Anura and class Amphibia of the phylum Chordata. The original distribution of the Bullfrog is to the east of the Rocky Mountains in the United States, in 30° to 40° north latitude area and southern Ontario and Quebec areas in Canada. It is the largest frog found in North America [2] and the adult frog is generally 8–12 cm long and can reach a maximum weight of 2 kg. Bullfrog breeding is mainly distributed in Guangdong, Fujian, Zhejiang, Jiangxi, Hainan, Anhui, Jiangsu, Hunan, Hubei, Sichuan and other southern regions [1]

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Conclusion