Viability Test of the Gourami Scales Collagen Extract (Osphronemus Goramy) on the Human Gingival Fibroblast Cells

Abstract

Background: Periodontal disease is a pathological inflammatory condition of the periodontal tissueswhich surround the teeth, including Human Gingival Fibroblasts (HGF). HGF regeneration is throughthe accelerating proliferation of tissue engineering therapy needs. Generally, the tissue engineering usesregenerative materials from cow or pig as the therapies, but these materials have some flaws. Thereby,this research aims to find the alternative materials regenerative tissue engineering scaffold collagen type1 derived from the gourami fish scales. This research is also conducted to test the viability of fish scalescollagen gourami against the Human Gingival Fibroblasts for 24 hours.Objective: To determine the concentration of fish scale collagen gourami which can maintain the viabilityof human gingival fibroblast cells for 24 hours.Method: HGF is taken from the healthy gingiva and planted in 96 well plates. Fish scales collagen gouramiwith a concentration of 0.32 mg/ml, 0.16 mg/ml, 0.04 mg/ml, 0.02 mg/ml and 0.01 mg/ml were added toeach well and incubated during 24 hours. MTT Assay is performed to see the viability of fibroblast cells.Results: The viability of HGF were increased after the addition of fish scales collagen gourami on theconcentration 0.32 mg/ml until 0.01 mg/ml. The viability of the cells after the addition of fish scale collagengourami was shown above 100%.Conclusion: Fish scales collagen gourami has the potential in tissue engineering and the concentration of0.01 mg/ml shows the highest viability of HGF.