Abstract
This study aimed to assess the viability, distribution and penetration of human gingival fibroblasts (HGFs) into three types of acellular dermal matrix (ADM) membranes. HGFs (HGF1-PT1 cell line) were seeded and cultured onto three types of ADM membranes, namely medium-thickness and thick Regen, SureDerm and one collagen matrix (Mucoderm). Viability and proliferation of HGFs on membranes were assessed at 24, 48 and 72 h and seven days using the methyl thiazolyl tetrazolium assay. Distribution and morphology of cells were assessed qualitatively at 24 h and seven days using a scanning electron microscope (SEM). Penetration of HGFs on day seven was assessed using a transmission electron microscope (TEM). Data were analyzed using two-way ANOVA. Viability of HGFs was not significantly different on the four types of membranes at 24 or 48 h (P > 0.05). At seven days, viability on SureDerm was higher than that in other groups and cell viability on thick Regen was less than that in other groups (P < 0.05). Qualitative analysis by SEM showed that HGFs on SureDerm had a more natural morphology and they were spindle-shaped, uniformly distributed and dense while on day seven, no cell was noted on thick Regen. TEM showed that at seven days, maximum penetration was noted into SureDerm while no cell penetration was noted into thick Regen. Viability, distribution and penetration of HGFs into SureDerm and Mucoderm were more favorable than into thick Regen.
Published Version
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