Abstract
The article presents the results of research on the choice of a stabilizer for strains "CP-65K" and "BIEMG-51" orthopoxviruses, which helps to preserve their biological activity during lyophilization, storage in various temperature and time conditions and transportation. To select a stabilizing medium that ensures the greatest preservation of the virus during lyophilization and subsequent storage, depending on the composition of protective media, temperature and shelf life, we prepared 3 samples of each vaccine preparation with stabilizing additives: I and V samples consisted of 5 % peptone and 3 % sucrose; II and VI samples - of 3 % peptone and 2 % sucrose; III and VII samples - from skimmed milk; IV and VIII samples - control. All protective media were added to the viral materials in a 1:1 ratio. As a result of the conducted studies, it was found that of the tested samples of stabilizing media, the most protective property for the strains «CP-65K» CPOX and "BIEMG-51" smallpox vaccine virus had a stabilizing medium consisting of a mixture of peptone (5 %) and sucrose (3 %) in the final concentration, which ensured the safety of vaccine strains for 12 months month. at a temperature of (2-8) °C and minus (40.0±1.0) °C without a significant decrease in their biological activity (6.50±0.14 lg TCID50/cm3 and 6.25±0.14 lg TCID50/cm3, respectively).
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