VetBiobank, Vetmeduni Vienna: A bioresource for clinical animal biospecimens

Ingrid Walter,Stefan Kummer,Melanie Stargardt,Monika Wieser,Stefanie Burger

doi:10.5334/ojb.60

Ingrid Walter, Stefan Kummer + Show 3 more

Open Access

https://doi.org/10.5334/ojb.60

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Abstract

The VetBiobank was established in 2007 as part of the VetCore Facility for Research at the University of Veterinary Medicine, Vienna. The primary objectives of the VetBiobank are (1) to achieve comprehensive collections of high quality animal samples to support research projects and (2) to provide biological reference material as controls e.g. for method verification. Currently, collections mainly contain tumor tissues and healthy reference tissues from cats and dogs, but also a small number of biospecimens from other diseases and other species, including macaque, horse, pig, and small companion animals. A total number of around 58000 individual samples are stored. The VetBiobank is partner of BBMRI.at (the Biobanking and BioMolecular resource Research Infrastructure Austria [1]), the Austrian national node of the European research infrastructure BBMRI-ERIC [2], comprising the only member with a focus on veterinary medicine. Funding statement: The VetBiobank is part of the central research facility of the University of Veterinary Medicine, which takes over personnel and running costs. Additionally, the VetBiobank was and is currently supported by two grants from the federal ministry of Austria to BBMRI.at (BMWFM GZ: 10.470/0016-II/3/2013, BMBWF GZ: 10.470/0010-V/3c/2018).

Highlights

(1) Bioresource Overview Project description The VetBiobank was established in 2007 as part of the technology platform VetCore, the central research facility of the University of Veterinary Medicine, Vienna, aimed to support research projects by state of the art technologies
In 2014 the VetBiobank became a member of the BBMRI.at consortium, which attributes great importance on the sample quality by controlling the pre-analytical sample management process
The scope of the VetBiobank has expanded by offering sample management of prospective samples assigned to certain study projects including tissue samples as well as body fluids

Summary

BIORESOURCE PAPER

The VetBiobank was established in 2007 as part of the VetCore Facility for Research at the University of Veterinary Medicine, Vienna. The primary objectives of the VetBiobank are (1) to achieve comprehensive collections of high quality animal samples to support research projects and (2) to provide biological reference material as controls e.g. for method verification. Collections mainly contain tumor tissues and healthy reference tissues from cats and dogs, and a small number of biospecimens from other diseases and other species, including macaque, horse, pig, and small companion animals. In 2014 the VetBiobank became a member of the BBMRI.at consortium, which attributes great importance on the sample quality by controlling the pre-analytical sample management process. Documentation of key data regarding the sample collection process (e.g. warm ischemia time, cold ischemia time, fixation time) to enable comparability of samples. Fresh frozen tissues, three kinds of sample types o ~ 1 × 0.5 cm piece of tissue, in a cryomold embedded in OCT o ~ 0.3 × 0.3 cm pieces in a 1.8 ml cryovial with RNAlater o ~ 0.3 × 0.3 cm pieces in a 1.8 ml cryovial without any additives (native)

Snap frozen in liquid nitrogen

Findings

Tumor types Adenocarcinoma Adenoma Ameloblastoma Chondrosarcoma Carcinoma