Abstract
Intracellular transport is powered by plus end-directed kinesins and minus end-directed cytoplasmic dyneins walking on microtubules. These motors are often simultaneously present on vesicular cargos resulting in bidirectional motion. How these cargos are trafficked to their cellular destinations despite frequent directional switches remains unclear. Various factors such as adaptor proteins, motor numbers, and microtubule-associated proteins (MAPs) can potentially bias the transport direction. To study the contribution of these factors individually, in a controlled environment, we reconstituted vesicular cargo motility by attaching purified KIF16B (Kinesin-3) and Dynein-Dynactin-BICD2 (DDB) to fluorescent large unilamellar vesicles (LUVs).
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