Abstract
Diapause is an important biological characteristic for many insect species to adapt to adverse environmental conditions and maintain the continuity of the race. Compared with the traditional hydrochloric acid or/and cold storage treatment methods, the artificial corona incubation technology of silkworm (Bombyx mori) eggs has many advantages including, the absence of pollution, easy operation and safety. However, this technology has not yet been applied in sericulture. In this study, we developed a novel artificial corona instrument to successfully disrupt the diapause of newly laid and refrigerated eggs from various Chinese and Japanese lineage silkworm strains. Subsequently, we invented a very early corona treatment (VECT) strategy to prevent the diapause of newly laid silkworm eggs within 4 h of oviposition. The hatching rates of the larvae were more than 95% in all diapause silkworm strains, which was comparable to the effect of the traditional HCl treatment strategy. In addition, we developed a combination strategy of VECT and pre-blastoderm microinjection and successfully created transgenic silkworms in various diapause strains. The results of the current study can aid in improving the corona artificial incubation technology and promote its application in sericulture.
Highlights
Diapause, a reversible developmental arrest, is an important physiological characteristic of insects to cope with periodic adverse environmental conditions and maintain ethnic continuity
The results revealed that the operating range of the corona treatment was a circular area with an average diameter of 41 ± 1.73 mm (n = 3), with the hatching rate close to 100%
Researchers from Japan have discovered that electric induction treatment can result in the artificial incubation of silkworm eggs; corona stimulation to terminate the diapause of silkworms gradually became a hot spot in Chinese sericulture research in the 1990s
Summary
A reversible developmental arrest, is an important physiological characteristic of insects to cope with periodic adverse environmental conditions and maintain ethnic continuity. Environmental conditions such as photoperiod (Emerson et al, 2009), temperature (Nibouche, 1998) and food quality (Wallace, 1970) trigger physiological responses that induce diapause in insects, and the internal conditions are mainly regulated by hormones (Nijhout, 1998; Sim and Denlinger, 2013). The dormancy of B. mori enables it to overcome severe environmental changes such as insufficient food supply and low temperatures (Yamashita and Hasegawa, 1985) It benefits the copulation of silkworms and ensures the reproduction of the offspring by synchronising populations (Yamashita and Hasegawa, 1985). Research on the mechanism of diapause and the release method of silkworms is of significance in the practice of silkworm seed production
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
