Abstract
We report a study of the repeatability as limited by instrumental imaging distortions in scanning-probe microscope (SPM) measurements of the heights of nominal 44 and 88 nm steps in calibration artifacts. By imaging the same series of locations on different days, we are able to distinguish sample variations from variations originating in the imaging process. In particular, the value and repeatability of the measured step heights are found to depend upon the algorithm used to infer the step height from the SPM image. The three general approaches tested are: a manual single-point method, which represents the most commonly used practice in the SPM community; a histogram-based method, which is available in commercially available SPM image-analysis software; and the polynomial step-function fit (PSFF), which explicitly removes image bow and sample tilt. Factors related to variations in the sample measured such as image size and location on the sample cause up to 10% variations in the step-height measurements. ...
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