Vertical distribution of microplastics in water bodies causes sublethal effects and changes in Daphnia magna swimming behaviour

Abstract

Plastic debris has been found to be ubiquitous in many aquatic ecosystems and is constantly accumulating, not only because more and more plastic is being rapidly released into the environment, but also because its slow degradation means it persists in the water. Some more buoyant plastics accumulate in the water column, whereas other heavier types sink to the bottom. Consequently, the presence of microplastics can threaten organisms living in the water column as well as those found in the benthic zone. In this study, the filter feeder Daphnia has been found to ingest microplastics as the particle diameter (< 30 µm) is within their edible particle size range and they are unable to differentiate between particles of different natures. Four different treatments were considered: food only; only microplastic particles; 50% food and 50% microplastic particles; neither food nor microplastics. Sinking microplastics have been found to decrease Daphnia magna individuals’ swimming velocity during vertical or cruising swimming trajectories, therefore demonstrating the sublethal effects microplastics have on this organism. In addition, microplastics decreased their body growth and survival rates. In cases with the presence of only microplastics, the swimming trajectories of Daphnia indicated the most serious stress experienced as individuals reversed vertical or cruising swimming trajectories to hopping and sinking movements. Therefore, Daphnia individuals in freshwater systems polluted by microplastics might take on the role of ingesting them and later on transporting them to deeper layer water column. In this way microplastics that would remain in the water column for a long time due to their buoyancy, might accumulate at the bottom of the water column.