VERIFICATION OF THE SCREENING METHOD FOR THE DETERMINATION OF STREPTOMYCINS RESIDUES IN HONEY SAMPLES

Abstract

The main field of activity of the National Reference Laboratory of Veterinary Drug Residues Control is the expert evaluation of honey safety based on the parameter of antimicrobial drug residues in honey bathes exported from Ukraine to European and American countries. The content of streptomycin and its derivative dihydrostreptomycin is one of the safety parameters for honey, which is investigated by enzyme-linked immunosorbent assay (ELISA). Since 2002, we have developed and proposed the clear scheme to control export honey batches on the content of residual amounts of these aminoglycoside antibiotics by means of Ridascreen® Streptomycin R3104 test kit from R-Biopharm (Germany).
 The test kit has been validated at 2.0 and 5.0 µg/kg taking into account the minimum required performance limit (MRPL) or the set reference point for action (RPA) according to European Commission Decision 2002/657/EC and 2021/808/EC. The fitness-for-purpose of the methods was confirmed based on the establishing of main validation parameters for screening methods (technical threshold and cut-off factor) using control (blank) honey samples and spiked with streptomycin standard solutions at the level of ½ MRPL by means of “added – found” approach. The preparation of samples is based on the preliminary purification by the method of solid-phase extraction using C18 columns, which facilitates the purification the sample extract from the influence of matrix components as well as the additional concentration of the analyte.
 The article also presents the results of the verification and validation of the Streptomycin NA01904H test kit from Kwinbon Biotechnology (China). The results of the assay of control (blank) honey samples and spiked with streptomycin standard solutions at the level of 2.0 and 5.0 μg/kg are given. Since the advantage of the approved method is the simplicity and rapidity of its performance, so we suggest using it in the laboratory practice of manufacturing and exporting companies.