Abstract
The influence of duration of washing and storage at different temperatures after treatment of barley kernels with ethyl methanesulfonate (EMS) and isopropyl methanesulfonate (iPMS) was investigated. Seedling height reduction was used to measure the effects of the alkylating agents. Without redrying and without storage the effect of EMS and iPMS were practically independent of post-treatment washing of the seeds. Redrying strongly enhanced the action of EMS, but had a relatively small effect on iPMS treated kernels. When using iPMS as a mutagen, a longer period of washing in order to avoid large aftereffects is therefore not necessary. The higher reaction rates of iPMS thus permits, in comparison with EMS, certain simplifications of the treatment scheme, and renders the compound useful mutagen in practical plant breeding. To the advantages of iPMS in this respect should be added the linear dependance of mutation frequency on concentration, observed in barley and E. coli . The action and decay of the two compounds in water and tissue at different temperatures is discussed.
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