Ventral horn synaptology in the rat

Abstract

The synaptology of the normal ventral horn of the rat was studied. Presynaptic boutons were classified as S (Spherical vesicles), F (flattened vesicles), and G (predominance of 700-1200 A granular vesicles). In addition, Cf, Cs, M, and T synaptic complexes were defined and quantitated. Synaptology was studied on alpha-motoneuron somata, alpha-motoneuron primary dendrites, peripheral dendrites and interneuron somata. In addition, organelles were quantified for the pre- and postsynaptic members of the synaptic complex. All counts were made on coded material and these data were analyzed statistically. Motoneuron somata had significantly more (P less than 0.01) F (58%) than S (33%) boutons. This was also the case for the motoneuron primary dendrite (P less than 0.01; F, 61%; S, 37%). The small dendrites had more (P less than 0.05) S (56%) than F (44%) boutons. More Cf bulbs (P less than 0.01) were found on motoneuron somata (9%) than on motoneuron primary dendrites (2%) or interneruon somata (3%). The C complex presynaptic bouton contained spherical (Cs) or flattened (Cf) synaptic vesicles which were attributed to the fixation employed. Cf bulbs were not observed on small dendrites. G bulbs were observed (less than 1%) only on small dendrites M bulbs were not observed on any postsynaptic structure. The boutons of the motoneuron primary dendrites (15% of total afferents) and peripheral dendrites (14% of total afferents) were frequently branched whereas there was significantly (P less than 0.01) less branching of boutons on motoneuron and interneuron somata. Small postsynaptic subsurface cisterns were associated with boutons of both the S and F type on all structures. In addition, these cisterns were observed in motoneuron somata (4%) and interneuron somata (2%) without an accompanying bouton. C postsynaptic organelles were observed in motoneuron somata (3%) and primary dendrites (1%) with an overlying neuroglial cell process and no presynaptic bouton. The synaptology of the rat ventral horn is comparable to that in the cat and monkey. However, M (R) and P bulbs were not observed in the rat. This could be due to the sampling method which indicated that synapses with less than 1% occurrence fall at the level of statistical resolution in quantitative electron microscopy. The presence of postsynaptic specialization usually associated with presynaptic boutons with no presynaptic component may be a reflection of the dynamics of normal bouton renewal in the rat ventral horn.