Abstract
Abstract : The replication of Venezuelan equine encephalitis (VEE) virus is completely inhibited at 44 C. The inhibited steps were analyzed by shifting the incubating temperatures up or down, and determining the rate and extent of infectious RNA synthesis, intact virus synthesis, and complement-fixing antigen formation during the shifts. The inhibition appears to be due to the presence of two temperature-sensitive steps involved in the synthesis of VEE virus in chick fibroblast cells. The first step of inhibition at 44 C occurs early in virus replication and can be completely reversed simply by returning cultures to 37 C. The time of inhibition appears to be some point between the time when the virus enters the cell and is uncoated and the beginning of viral RNA synthesis. The second temperature-sensitive step in VEE virus synthesis is irreversible and occurs at a point after the synthesis of viral RNA and before the formation of virus structural protein as measured by complement-fixing antigen.
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