Abstract
A fish cell line derived from rainbow trout gonads (RTG) has been shown to support the proliferation of 2 arboviruses, Venezuelan equine encephalitis (VEE) virus and Eastern equine encephalitis (EEE) virus, at 22°C. EEE virus was more cytopathogenic for RTG cultures than VEE virus, thus making it feasible to distinguish between the two. A persistent infection was obtained with VEE virus for as long as cultures were carried, but not with EEE virus. Both viruses multiplied in chick fibroblast (CF) cells at 22°C to slightly higher titers than in the fish cell line and both viruses caused a cytopathogenic effect in CF cells. L cells failed to support the growth of either virus at 22°C.
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