Abstract
Recently, the rat mesentery culture model was established as a tool for investigating multi‐cellular dynamics involved in angiogenesis. Cultured tissues displayed intact blood and lymphatic microvascular networks. The objective of this study was to determine whether VEGF‐C induces lymphangiogenesis in this model. Mesenteric tissue windows were harvested from adult male Wistar rats and cultured for 5 days in either serum‐free minimum essential media (MEM) or MEM supplemented with VEGF‐C (100 ng/ml). Tissues were then labeled for PECAM and LYVE‐1. VEGF‐C treatment caused a significant increase in the number of lymphatic sprouts per lymphatic network area compared to MEM controls (p < 0.01). Lymphatic sprouts were observed along the hierarchy of intact lymphatic networks and were PECAM and LYVE‐1 positive. These results suggest the rat mesentery culture model can be used to investigate the interrelationships between lymphangiogenesis and angiogenesis in a controlled environment.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call