Abstract
Arginine-vasopressin (AVP) neurons exist in the hypothalamus, a major region of the diencephalon, and play an essential role in water balance. Here, we established the differentiation method for AVP-secreting neurons from human embryonic stem cells (hESCs) by recapitulating in vitro the in vivo embryonic developmental processes of AVP neurons. At first, the differentiation efficiency was improved. That was achieved through the optimization of the culture condition for obtaining dorsal hypothalamic progenitors. Secondly, the induced AVP neurons were identified by immunohistochemistry and these neurons secreted AVP after potassium chloride stimulation. Additionally, other hypothalamic neuropeptides were also detected, such as oxytocin, corticotropin-releasing hormone, thyrotropin-releasing hormone, pro-opiomelanocortin, agouti-related peptide, orexin, and melanin-concentrating hormone. This is the first report describing the generation of secretory AVP neurons derived from hESCs. This method will be applicable to research using disease models and, potentially, for regenerative medicine of the hypothalamus.
Highlights
Arginine-vasopressin (AVP) neurons exist in the hypothalamus, a major region of the diencephalon, and play an essential role in water balance
Wataya et al reported a method for differentiating hypothalamic neurons from mouse embryonic stem cells[8]
We aimed to establish a method to differentiate hypothalamic neurons from human embryonic stem cells (hESCs) based on the protocol of Wataya et al.[8]
Summary
Arginine-vasopressin (AVP) neurons exist in the hypothalamus, a major region of the diencephalon, and play an essential role in water balance. Other hypothalamic neuropeptides were detected, such as oxytocin, corticotropinreleasing hormone, thyrotropin-releasing hormone, pro-opiomelanocortin, agouti-related peptide, orexin, and melanin-concentrating hormone This is the first report describing the generation of secretory AVP neurons derived from hESCs. This is the first report describing the generation of secretory AVP neurons derived from hESCs This method will be applicable to research using disease models and, potentially, for regenerative medicine of the hypothalamus. If CDI is accompanied by dysfunction of osmoreceptors, patients can become adipsic and show life-threatening dehydration[3] To overcome these problems, regenerative medicine is warranted. We aimed to establish an in vitro-method for the functional differentiation of AVP neurons that secret AVP using human embryonic stem cells (hESC)
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