Abstract
Our lab demonstrated that VBMT, the microsurgical transplantation of a femur with its vascular pedicle, can induce immune tolerance across a semi‐allogeneic barrier without graft versus host‐disease. We hypothesized that VBMT alone is potentially superior to cellular stem cell transplantation and sufficient to induce normal repopulation of the spleens of XRT rat recipients.Lewis Brown Norway rats received 9 Gy radiation. Rats then received either a conventional cellular (cBMT, n=5) or VBMT (n=4) bone marrow transplant from Lewis donors. Control groups were: non‐transplanted, non‐irradiated (CT, n= 5) or XRT only (n= 4). Stained histologic sections of spleens were analyzed with digital image analysis (DIA).XRT rats died 9±1 days post irradiation. Other groups were sacrificed: cBMT at 110 days; VBMT at 102 ±1 days. Compared to CT, XRT spleens showed white pulp atrophy; cBMT had less white pulp but appeared hypercellular and VBMT spleens appeared normal. DIA results can be seen in the table below:VBMT is capable of reconstituting spleens of XRT semi‐allogeneic recipients similar in appearance to normal rats. This model has clinical relevance for both stem cell transplantation and solid organ transplantation, in that VBMT induces chimerism and tolerance.
Published Version
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