Abstract
The current chapter was carried out in an attempt to study the transmission of Ralstonia solanacearum, the causal agent of potato brown rot, through field weeds, survey, isolation, identification and varietal differences in their relation to brown rot disease resistance in potato under Egyptian condition. The bacterial wilt problem is a major constraint for vegetable growers, especially potato and tomato farmers in many regions of the world. The number of weed varieties as hosts for R. solanacearum is very great. It causes disease at least of 200 different plant species, including herbaceous plants, shrubs and trees. There is increasing evidence of hosts which under certain condition act as latent or symptomless carriers of infection. Isolates of R. solanacearum from weeds and other plant species were identified as race 3 biovar 2 using morphological, physiological and biochemical tests. Also advanced Immunofluorescence Antibody Stains (IFAs), Polymerase Chain Reaction (PCR) and Real-time PCR (Taq-Man) techniques were applied and proved that the isolates are related to race 3 biovar 2 of R. solanacearum. Potato cultivars showed various levels of disease resistance against R. solanacearum. Spunta and Pyrnadet cvs. were more sensitive; Nicola cv. was moderate sensitive; Mondial cv. was moderate resistant, while Lady Roasita was resistant to the pathogen. Plant extracts from Corchorus olitorius, Solanum. nigrum, Ricinus communis and Portulaca oleracea appeared to be more effective against R. solanacearum.
Published Version
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