Abstract
Simple SummaryLipin 1 plays an important role in lipid metabolism. It not only acts as a phosphatidate phosphatase and directly participates in the synthesis of glycerol and osteolipid, but also acts as a transcriptional co-activator to indirectly regulate the expression of genes related to lipid metabolism. In livestock species, variation in the lipin 1 gene (LPIN1) has been reported in pigs, chickens, and dairy cows, but has not been investigated in sheep, and little is known about whether it might affect production traits in this globally important meat-producing species. In this study, we used polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) analyses to search for variation in ovine LPIN1, and its effects on production and carcass traits were investigated in New Zealand Romney sheep. The results suggest that ovine LPIN1 is variable and it may have value as a genetic marker for improving meat production and carcass traits.Lipin 1 plays an important role in lipid metabolism. In this study; we searched for variation in the ovine lipin 1 gene (LPIN1) in three gene regions (a 5′ non-coding region; a region containing an alternatively spliced exon in intron 4; and a region containing coding exon 6) using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) analysis. The greatest amount of alleles was found in coding exon 6; with five sequences being detected. The effect of variation in this exon was investigated in 242 New Zealand Romney lambs derived from 12 sire-lines. The presence of variant E3 was associated with a decrease in birth weight (p = 0.005) and the proportion of leg yield (p = 0.045), but with an increase in hot carcass weight (p = 0.032) and the proportion of loin yield (p = 0.014). The presence of variant B3 was associated with an increased pre-weaning growth rate (p = 0.041), whereas the presence of variant C3 was associated with an increase in shoulder yield (p < 0.001). These results suggest that ovine LPIN1 variation may have value as a genetic marker for improving meat production and carcass traits.
Highlights
Lipin 1 was first identified from the fatty liver dystrophy mouse [1]
Four Polymerase Chain Reaction (PCR)-SSCP banding patterns were detected in the 50 non-coding region, two banding patterns were detected in the LPIN1β-spliced exon region, and five banding patterns were found in the coding exon 6 region (Figure 1)
This study explored genetic variation in three regions of ovine lipin 1 gene (LPIN1), and the effect of variation in coding exon 6 was investigated on some key growth and carcass traits in New Zealand (NZ) Romney sheep
Summary
Lipin 1 was first identified from the fatty liver dystrophy (fld) mouse [1]. It is a member of the lipin family of proteins, and it is involved in animal lipid metabolism and its regulation. Lipin 1 is normally present in adipose tissue, skeletal muscle and heart tissue [1,2]. It has been demonstrated that lipin 1 plays an important role in the nucleus, both through involvement in the regulation of peroxisome proliferator activated receptor gamma (PPARG)-mediated increases in adipogenic gene expression, and as an enzyme in the triglyceride synthesis pathway [3]. Increased lipin 1 expression in either adipose tissue or skeletal muscle results in increased adiposity [4]. A lack of lipin 1 results in the metabolic abnormalities observed in fld mice [1]
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