Abstract
350bp segments of the flagellin gene of five isolates of Borrelia burgdorferi sensulato were compared to B. burgdorferi B-31 and B. andersonii using polymerase chain reaction and heteroduplex mobility assay. Borrelia andersonii and B.burgdorferi were 99.1% similar in mobility. All of the isolates, MQD-1, 2, 3, 5, and 6, were from one farm in southeastern Missouri; four were isolated from Ixodes dentatus and one (MOD-6) was isolated from Amblyomma americanum. The amplified product of MOD-2 was identical (100%) to B. andersonii; MOD-1 and MOD-3were very similar (99.8%) to B. andersonii, and MOD-5 was 99.7% similar to B.burgdorferi B-31. MOD-6 was the most divergent of the Missouri isolates from both B-31 (98.5%) and B. andersonii (99.3%). This study demostrates the usefulness of the heteroduplex mobility assay as a tool for rapidly identifying genetic variation among Borrelia strains.
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