Abstract

The hydrolytic activities of 10 strains of Histoplasma capsulatum and 10 of H. duboisii were studied on gelatin, tyrosine and urea substrates. The data revealed that species specific differences in enzymatic activity for these substrates did not occur. Consequently, these tests cannot be used to separate the 2 species. Definitive identification of H. duboisii cultures still requires that animal inoculation be used to demonstrate the large yeast form characteristic of this species. Las actividades hidrolíticas de 10 cepas de Histoplasma capsulatum y 10 cepas de H. duboisii fueron investigadas con substratos conteniente gelatina, tirosina y urea. Los resultados revelan que no es posible diferenciar las cepas de H. capsulatum de las cepas de H. duboisii por medio de sus actividades hidrolíticas. Consiquientemente, no es posible con esta prueba distinguir los dos especies. Por causa eso, es aun necesario identificar cepas de H. duboisii por medio de inoculación de animales para demostrar las formas grandes de levadura características de este hongo.

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