Abstract
BackgroundPersistent infection of high-risk human papillomaviruses 16 (HPV16) has been considered as the leading cause of cervical cancer. In this study we assessed HPV16 sequence variation and genetic diversity of HPV16 variants in cervical cancer in Uigur women in Xinjiang, China. We analyzed the nucleotide sequences of the open reading frames of E6 and E7, and part of the open reading frames of L1 of HPV16 in Uigur women.MethodsBiopsies of histologically confirmed HPV16 infections with cervical cancer were obtained from 43 Uigur women in Xinjiang, China. E6, E7 and L1 genes of HPV16 of all samples were amplified and sequenced; the sequences were used in phylogenetic analysis of HPV16 variants.ResultsOur analysis revealed nine nucleotide changes in E6 (five changes), E7 (one change) and L1 (three changes) gene. The most frequently observed variations were T350G (79.1 %). One variation T295G (D64E) at E6 were detected in 6 cases (KT959536, KT959542, KT959546, KT959550, KT959553, KT959558). Deletion (464Asp) along with insertion (448Ser) were observed in L1 (100 %). Most variants were European lineage (97.7 %); only one belongs to Asia variants with common T178G (D25E) in E6 and A647G (N29S) in E7.ConclusionThe most prevalent HPV16 variants in the Uigur women we studied were of the European lineage. Our results indicate that HPV16 European lineage may serve as a harmful factor associated with the development and progression of cervical cancer.Electronic supplementary materialThe online version of this article (doi:10.1186/s13027-016-0089-2) contains supplementary material, which is available to authorized users.
Highlights
Persistent infection of high-risk human papillomaviruses 16 (HPV16) has been considered as the leading cause of cervical cancer
We investigated the genetic variation of HPV16 in Uigur women in Xinjiang
Our results show that HPV16 variant of European lineage is the most common type in Uigur women in Xinjiang, which is markedly different from elsewhere in China
Summary
Persistent infection of high-risk human papillomaviruses 16 (HPV16) has been considered as the leading cause of cervical cancer. In this study we assessed HPV16 sequence variation and genetic diversity of HPV16 variants in cervical cancer in Uigur women in Xinjiang, China. Cervical cancer was the third most common cancer among women in the world, with 527,624 new cases and 265,653 deaths in 2012 [1]. Persistent infection by high-risk HPV16, has been recognized as a critical etiological factor for cervical cancer [4], which is present in over half of invasive cervical cancer cases worldwide [5, 6]. Previous studies demonstrated that HPV16 variants increased the risk for progression to cervical cancer [8,9,10], but the roles of HPV16 genetic variation are poorly understood
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