Abstract
Abstract Eggs (0-3 d old) placed in open petri dishes or peroxide penetrable bags and last instar larvae placed in plastic bags were fumigated with vapor-phase hydrogen peroxide. Half of the bags contained 5 ‘Carmel’ almond nutmeats. The bags used for fumigation were constructed of Poly/Tyvek (American Sterilizer Co.) and were 8 cm wide by 13 cm long. Fumigations were conducted in a VHP Dental Sterilizer (fumitorium) manufactured by American Sterilizer Co., Apex, NC. One hundred eggs or ten larvae were placed per container and treated with a dose of 8.9 °9.0 g of 35% liquid H2O2 injected on either cycle I (deep vacuum 32mm Hg) or cycle II (shallow vacuum ca. 400mm Hg), both cycles employed repeated injections of 40 micro s duration over the treatment period. Preliminary testing indicated that cycle I produced greater mortalities than did treatment under cycle II, therefore, cycle I was employed for the reported tests. Each treatment required 30 minutes and chamber temperature rose to 43-47°C. Controls were treated as described except water was substituted for hydrogen peroxide. Each test was replicated three times.
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