Vanadium-resistant endophytes modulate multiple strategies to facilitate vanadium detoxification and phytoremediation in Pteris vittata.

Abstract

Vanadium (V) contamination of soils poses potential risks to humans and ecosystems. This study was conducted to evaluate the effects of endophyte-assisted phytoremediation and to determine the mechanisms involved in V detoxification and plant growth promotion. Results showed that the endophytic bacterium Serratia marcescens PRE01 could successfully colonize the roots and increase the total V uptake of Pteris vittata by 25.4 %, with higher plant biomass and V accumulation in roots. Endophyte inoculation significantly improved the secretion of phytic, malic, and oxalic acids and accelerated FeVO4 dissolution and subsequent Fe and V uptake in the rhizosphere. Under V stress without inoculation, V removed by shoot uptake, root uptake, and root surface adsorption accounted for 21.76 %, 42.14 %, and 30.93 % of the total V removal efficiency, respectively. To detoxify excess V, PRE01 effectively strengthened the adsorption of V on the root surface, with an increase in its contribution to the total V removal efficiency from 30.93 % to 38.10 %. Furthermore, beneficial endophytes could alleviate oxidative damage caused by V stress by reinforcing the plant antioxidant system and promoting V(V) reduction in root tissues. These findings clearly reveal that inoculation with endophytes is a promising method for modulating multiple strategies to enhance the phytoremediation of V-contaminated soils.