Abstract
The effect of different vanadium compounds on proliferation and differentiation was examined in osteoblast-like UMR106 cells. Vanadate increased the cell growth in a biphasic manner, the higher doses inhibiting cell progression. Vanadyl stimulated cell proliferation in a dose-responsive manner. Similar to vanadate, pervanadate increased osteoblast-like cell proliferation in a biphasic manner but no inhibition of growth was observed. Vanadyl and pervanadate were stronger stimulators of cell growth than vanadate. Only vanadate was able to regulate the cell differentiation as measured by cell alkaline phosphatase activity. These results suggest that vanadium derivatives behave like growth factors on osteoblast-like cells and are potential pharmacological tools in the control of cell growth.
Highlights
Osteoblasts are important cells involved in bone formation
The addition of insulin, IGF-I and bovine GH to UMRl06 cells in serum-free media produced a significant increase in cell proliferation after 24 h of incubation
These results showed that the growth of osteoblast-like UMRl06 cells is regulated by different hormones and growth factors
Summary
Osteoblasts are important cells involved in bone formation. Their activity is regulated by several systemic hormones (growth hormone, GH; insulin; glucocorticoids; parathyroid hormone, PTH; vitamin D) and local factors like transforming growth factor (TGF), fibroblast growth factor (FGF), insulin-like growth factors (lGFs), plateletderived growth factor (PDGF) Il-31. Systemic hormones can act directly on the bone or by inducing the secretion ofcytokines and growth factors [4]. It has been found that human bone cells in culture produce a number of growth factors, many of which are known to be stored in human bone, including IGF-1, IGF-Il, TGF-p and PDGF [5]. The hormonal action is mediated by cell surface specific receptors identified in osteoblasts in culture [G{]. Several ions (Ca, Zn,p,Mn, Cu, V) modulate the activity of the bone cells [9, l0]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
