Valorization of agro-industrial wastes into polyhydroxyalkanoates-rich single-cell proteins to enable a circular waste-to-feed economy

Abstract

Recovering and converting carbon and nutrients from waste streams into healthy single-cell proteins (SCPs) can be an effective strategy to address costly waste management and support the increasing animal feed demand for the global food supply. Recently, SCPs rich in polyhydroxybutyrate (PHB) have been identified as an effective biocontrol healthy feed to replace conventional antibiotics-supplemented aquaculture feed. PHB, an intercellular polymer of short-chain-length (SCL) hydroxy-fatty acids, is a common type of polyhydroxyalkanoates (PHA) that can be microbially produced from various organics, including agro-industrial wastes. The complex chemical properties of agro-industrial wastes might produce SCPs containing PHA with SCL and/or medium chain-length (MCL) hydroxy-fatty acids. However, the effects of MCL-PHA-containing SCPs on aqua species' health and disease-fighting ability remains poorly understood. This study investigated the feasibility of producing various PHA-containing SCPs from renewable agro-industrial wastes/wastewaters, the effectiveness of SCL- and MCL-PHA as biocontrol agents, and the effects of these PHA-rich SCPs on the growth and disease resistance of an aquaculture animal model, brine shrimp Artemia. Zobellella denitrificans ZD1 and Pseudomonas oleovorans were able to grow on different pure substrates and agro-industrial wastes/wastewaters to produce various SCL- and/or MCL-PHA-rich SCPs. Low doses of MCL-fatty acids (i.e., PHA intermediates) efficiently suppressed the growth of aquaculture pathogens. Moreover, MCL-PHA-rich SCPs served as great food/energy sources for Artemia and improved Artemia's ability to fight pathogens. This study offers a win-win approach to address the challenges of wastes/wastewater management and feed supply faced by the aquaculture industry.