Abstract
Polyhydroxyalkanoates (PHA)s are microbially produced polyesters that are biodegradable and have a wide variety of uses. PHAs can be divided into three groups dependent on the size of the monomers incorporated into the polymer. Short-chain-length (SCL) PHAs are composed of C3-C5 monomers and are thermoplastic in nature, while medium-chain-length (MCL) PHAs are composed of C6-C14 monomers and are elastomeric in nature. SCL-MCL PHAs are composed of monomers C3-C14 in length and where the properties of the polymer vary based on the mol% composition of SCL and MCL monomers. This study compares various monomer-supplying pathways in recombinant Escherichia coli used for PHA production from non-related carbon sources to supply SCL and MCL PHA monomers. In addition, data describing the composition and thermal properties of polymers produced in recombinant E. coli using engineered PHA synthases and fatty acid biosynthesis enzymes are presented.
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