Abstract
Definite detection of MRSA is important for proper treatment. The purpose of the present study was to measure the validity of cefoxitin disc diffusion test for the detection of methicillin-resistant Staphylococcus aureus. This cross-sectional study was carried out in the Department of Microbiology and Immunology at Bangabandhu Sheikh Mujib Medical University, Dhaka from January 2010 to December 2010 for a period of one (01) year. S. aureus isolates were collected from different clinical samples including wound swab, pus, blood, urine, tracheal aspirate, throat swab, aural swab etc. Staphylococcus aureus (S.aureus) were isolated and confirmed by staining, biochemical tests. Routine antimicrobial susceptibility testing was performed cefoxitin discs diffusion test. PCR was performed for detection of the mecA gene for MRSA. Out of the 22 suspected MRSA isolates 19 were mecA positive by PCR and all of them 19(100.0%) were resistant to cefoxitin disc diffusion. The comparison of oxacillin and cefoxitin resistance and presence of mecA gene by PCR showed that out of 22 suspected MRSA isolates 19 were mecA positive by PCR and all the 19 (100.0%) showed resistance to cefoxitin disc diffusion. The sensitivity and specificity of cefoxitin disc diffusion were 100.0% and 100.0% respectively. Cefoxitin disc diffusion test has high sensitivity and specificity for the detection of MRSA.
Highlights
Antibiotic resistant pathogens constitute an important and growing threat to the public health[1]
The present study was undertaken to detect the Methicillin-resistant S. aureus (MRSA) by cefoxitin disc diffusion method and to compare with polymerase chain reaction (PCR) for the detection of mecA gene for MRSA
Conventional PCR was performed to detect mecA gene of 22 suspected S. aureus strains resistant to cefoxitin by disc diffusion at the Molecular Laboratory in the Department of Microbiology and National Forensic DNA Profiling Laboratory of Dhaka Medical College, Dhaka
Summary
Antibiotic resistant pathogens constitute an important and growing threat to the public health[1]. Antibiotic resistance occurs when a microbe acquires a gene or alters its structural components, which allows the microbe to inactivate the antibiotic or nullify its antimicrobial activity[2]. This may occur as a spontaneous, genetic mutation or involve acquisition of a genetic element such as plasmid, transposon, integron or gene cassette[3]. Quick and reliable identification procedure is required to obtain information on the MRSA isolates[5]. This measure allows faster implementation of appropriate control measures.
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