Validity of B1 Gene in Diagnosis of Prenatal and Postnatal infection with Toxoplasma gondii

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Toxoplasma gondii is one of the most frequent parasite all over the world. Most patients are asymptomatic, except in immunosuppressed individuals and pregnant women , which can be seriously injured. Prenatal diagnosis should be made rapidly in view of the fact that treatment of the mother can reduce fetal sequelae. The present study was used Rapid test as a serological method to detected IgG and IgM antibodies titers and the Nested polymerase chain reaction technique (nPCR) in 74 samples of blood were collected from pregnant women whom with history of abortion. The aborted cases were 33 out of 74, while 41cases were reach birth, 15 cases were early birth ( 29-36 week) period and 26 cases were reach birth with normal pregnancy period ( 37-42 week). The result of Rapid test reveled that IgM and IgG were positive in pregnant women 29/ 74 and 47/74 respectively , while the nested polymerase chain reaction technique (nPCR) result showed 36/74. The Nested polymerase chain reaction technique (nPCR) was also done on the ( 41) newborn babies. The total number of newborn babies with congenital anomalies were 16 and the positive result of (nPCR) in newborn showed 17. The validity of B1 gene in prenatally toxoplasmosis showed the sensitivity, specificity, positive productive value and negative productive value were (91%, 81% ,78% and 100%) respectively, while the sensitivity, specificity, positive productive value and negative productive value in postnatally were (88%, 83%, 94% and 96%) respectively. The aim of study was the early finding congenital toxoplasmosis of maternofetal transmission, for early treatment to avoid unwanted sequelae using nPCR technique in support of early diagnosis T. gongii in the prenatal of congenitally and to evaluate these results to the postnatal infection.