Validation Study on Spectrophotometric Measurement of Ethanol from Beer and Non-Alcoholic Beer Samples Distilled by Micro Steam Distillation Method

Abstract

The method validation technique for determining the ethanol percentage (v/v) in beer and non-alcoholic beer is presented in this study. The underlying idea is to use the micro water vapor method to distill the ethanol from beer and then use the spectrophotometric method, which uses the oxidation of sodium dichromate to measure the amount of ethanol. The Harmonized Guidelines for Single-Laboratory Validation of Methods of Analysis were used to validate the analytical method that we provided. The following aspects of the method were assessed: precision, recovery, linearity, measuring range, detection and quantification limitations, method detection limit, and measurement uncertainty. The following were the limits of detection (LOD) and quantification values (LOQ), and the method detection limit (MDL): ethanol, 0.04%, 0.05%, and 0.15. For both repeatability and within-laboratory reproducibility, the relative standard deviation values were less than 2.36 and 4.12%, respectively. The spiked samples had recovery rates ranging from 97% to 102%. These findings fulfilled the minimal performance standards set forth in AOAC Official Methods of Analysis Appendix F: Guidelines for Standard Method Performance Requirements. As a result, the process can be used for the regular analysis of ethanol in the beer and non-alcoholic beer under study.