Abstract
The principle purpose of this study was to investigate the frequency distribution of the short tandem repeat system FXIIIB in a sample of individuals from Tuscany (Central Italy). Samples (204), taken from donors residing in the area of Florence, Prato and Pistoia, were analysed by PCR and vertical denaturing polyacrylamide gel electrophoresis. A sensitivity study was also undertaken and the method was optimised for the study of forensic samples. The research resulted in the identification of 5 alleles and 11 genotypes. The system was tested for Hardy–Weinberg equilibrium and the Central Italian population was compared with populations from Northern Italy and Austria. Moreover, we introduced the use of this microsatellite in an automatic platform, in combination with other STRs in a new multiplex amplification system. An infrared automatic DNA sequencer was used to analyse this marker. The introduction of this marker in our protocol could be useful to resolve particular intriguing cases of forensic interest, as for example incest or deficitary paternity/maternity tests, in which additional loci must be investigated.
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