Abstract
In order to discuss that whether the methods of total aerobic microbial count and absence bacterial test were appropriate in microbial limit tests for health foods hygiene. As the requirement of microorganism limits test and validation on the Chinese Pharmacopoeia 2010, the microbial examination method of two kinds of health foods has been validated. It is found that vitamin B was antibacterial for <em>Bacillus subtilis</em> and <em>Aspergillus niger</em>. The results indicated the method in foods microbial limit test GB/T4789-2010 were not suitable for the microbial limit tests of health foods. It is suggested that setting more reasonable method by validation as well as referring to the Chinese Pharmacopoeia 2010.
Highlights
With the development of economy and society, consumer demands in the field of food production have changed considerably
Samples: Protein powder and vitamin B were purchased from the local supermarket
Validation of method: The microbial limit test method referring to the Chinese Pharmacopoeia 2010 were performed three times independently in parallel experiments
Summary
With the development of economy and society, consumer demands in the field of food production have changed considerably. Strain: Escherichia coli [CMCC B44102], Bacillus subtilis [CMCC63501], Staphylococcus aureus [CMCC 26003], Streptococcus hemolytic-β [CMCC32210], Aspergillus niger [CMCC98003], Shigella flexneri [CMCC51572] and Salmonella [CMCC50094] were from Guangdong provincial institute of microbial culture collection center. Validation of method: The microbial limit test method referring to the Chinese Pharmacopoeia 2010 were performed three times independently in parallel experiments.
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