Abstract
Monitoring circulating progesterone concentration (P4) is an important component of basic and applied reproduction research and clinical settings. Radioimmunoassay (RIA) is considered the gold standard method for measuring P4 in sheep blood, but it generates radioactive waste, has low throughput and requires licensed facilities. Immulite® 2000XPI (Siemens) is a fully automated immunoassay system marketed for human use to measure concentrations of different analytes including P4. Our objectives were therefore to: 1) validate sheep serum P4 measurement with the Immulite 2000XPI; and 2) characterize the analytical performance of the Immulite 2000XPI P4 immunoassay (IPI) across the reportable concentration range.
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