Abstract
Leaf tissue from 10 Buxus spp. cultivars were collected and evaluated for differences in isozyme and random amplified polymorphic DNA (RAPD) markers. In addition, samples of named cultivars were collected from multiple locations to estimate the degree of cultivar fidelity across locations. In general, isozymes were highly polymorphic among cultivars. Aconitase, malate dehydrogenase, aminoaspartate transferase, and phosphoglucose isomerase were the most useful isozymes to distinguish among the selected clones. Good quality DNA was readily extracted from leaf tissue using a standard CTAB procedure. RAPDs were generated from polymerase chain reactions using a random set of primers. Of 200 primers that were screened, 20 gave reproducible and polymorphic patterns that could be used to distinguish among the boxwood cultivars. In general, cultivar fidelity among commercial nurseries was fair. Biochemical analyses could be used to resolve problems with cultivar identity once baseline information is collected.
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