Abstract
Abstract : The goals of the first year of the funding period were to develop methods for the analysis of FFPE (formalin-fixed paraffin-embedded) prostate cancer biopsy tissue in order validate the accuracy of a stroma-based classifier for diagnosis of prostate cancer using FFPE anatomically negative biopsies. The methods that needed to be developed were PCR primers that operate on RNA retrieved from FFPE prostate cancer tissue and to apply the primers as a microarray to accurately detect RNA corresponding to the primer targets. Proof-of-principal for these goals has been achieved.
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