Abstract
Objective: The purpose of this study was to obtain a valid analytical method for determining the level of 2,5-hexanedione in the urine of oil industry workers.
 Methods: Gas Chromatography (GC) was employed to analyze 2,5-hexanedione in the urine. The analysis was done using HP-5 (Crosslinked methyl siloxane) capillary columns 30 m x 0.320 mm long, film thickness 0.25 μm. The temperature of the detector temperature was 300 °C, and the injector temperature was 250 °C. The helium gas flow rate was 2 ml/min. The detector was Flame Ionization Detection (FID). Parameters of system suitability test and validation were obtained.
 Results: This study results that the method of analysis 2,5-hexanedione in urine by Gas Chromatography (GC) confirm the requirements of the validation method with a linearity was 0.99963, accuracy was in the range of 99.16% to 114.13%, the precision with % coefficient of variation was 1.65% to 5.16%, % coefficient variation of specificity was 0.027%, limit of detection was 0.054 μg/ml and limit of quantification was 0.18 μg/ml.
 Conclusion: The proposed GC method meets the acceptance criteria of validation parameters and can be applied for routine analysis.
Highlights
Population growth was increasing rapidly. It led to the birth of the industrialization era
The tools used in this study were a set of Gas Chromatography (GC) (Agilent Technologies®) with Flame Ionization Detector (FID), crosslinked methyl siloxane, centrifugation (Eppendorf AG®), analytical balance scale (Mettler Toledo®), spatel, glass vial (Agilent Technologies®), vortex (Digisystem®), flask, test tube, micropipette 10-100 μl and 100-1000 μl (ACURA 825®), and glass tools commonly used in Analytical Laboratory
The chromatographic system was optimized by injecting 2 μg/ml standard under the following conditions [12]: Columns: HP-5 (Crosslinked methyl siloxane) capillary columns, 30 m x 0.320 mm long, film thickness 0.25 μm, detector temperature 300 °C, injector temperature 250 °C, column temperature was programmed from 30 °C to 325 °C
Summary
It led to the birth of the industrialization era. In this era, the development of science and technology was more advanced. One impacts of science and technology is the use of chemicals in many work processes. Various chemicals are widely used in industries, such as food additives, pesticides, metals and compounds, as well as various organic chemicals including organic solvents [1]. One of the most widely used organic solvents in the industry is hexane
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