Abstract
Objective: The purpose of this research was to optimize and validate a method for measuring the levels of diallyl disulfide (DADS) and diallyl trisulfide(DATS) in garlic and single clove garlic.Methods: The analysis was performed using gas chromatography (GC) equipped with an HP-1 column and a flame ionization detector. The initialcolumn temperature was set at 140°C and increased at 1°C/min to 180°C. The injector and detector temperatures were set to 200°C, the carrier gasflow rate was 0.80 mL/min, and the injection volume was 1.0 μL. The optimized conditions of analysis were then validated which included selectivity,linearity, accuracy, precision, limit of detection (LOD), and limit of quantification (LOQ).Results: Using the validated assay and a concentration range of 0.5–20 μg/mL, the coefficient of correlation (r) for DADS was 0.9999 and the LODand LOQ for DADS were 0.3063 μg/mL and 1.0210 μg/mL, respectively. Using the validated assay and a concentration range of 0.5–20 μg/mL, thecoefficient of correlation for DATS was 0.9999 and the LOD and LOQ for DATS were 0.1986 μg/mL and 0.6621 μg/mL, respectively. The percentage ofrecovery was in the range of 98.05–101.76% and coefficient of variation ≤ 2%.Conclusion: This GC method accurately measures the levels of DADS and DATS in garlic.
Highlights
In Indonesia, garlic is an important farming commodity because it has various benefits [1]
Validation methods The assay was validated based on assay selectivity, linearity, limit of detection (LOD) and limit of quantity (LOQ), accuracy, and precision parameter
Analysis condition optimization The run conditions were optimized to obtain conditions that would result in relatively short retention times and good separation
Summary
In Indonesia, garlic is an important farming commodity because it has various benefits [1]. Garlic is seen as an important plant due to its benefits for use as a raw material for culinary purposes and its use in traditional and modern medicine [2]. Garlic has been demonstrated to have antioxidant, anticancer, and cardioprotective effects [3]. Due to all effects for the health related to garlic consumption, this plant is considered as one of the important remedies to improve health [5]. Organosulfur compounds are important to the bioactivity of garlic, including its derivative products. Smell, and pharmacological activity of garlic are related to its organosulfur compound [6]
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