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Abstract
ABSTRACT Introduction: Automated hematology analyzers are able to produce low platelet counts with great precision and accuracy. However, these analyzers may produce erroneous counts due to the presence of interferences. Therefore, additional methodologies are required to confirm platelet counts, such as blood smear and a direct count, using the Neubauer chamber. Objective: To validate the reliability estimate produced by the G&S method. Material and method: One hundred and fifty platelet counts were analyzed in the hematology and emergency laboratories of the Hospital Nacional Docente Madre-Nino San Bartolome, in Lima, Peru, by four methodologies: one optical platelet count (PLTO), one indirect blood smear count (Indirect), and two direct counts (Direct and G&S). Descriptive and inferential statistics were used to compare the groups. Results: A significant difference was observed in the distribution of the four methodologies and, after applying the post-hoc analysis, a similarity between the PLTO and G&S methods was found. Also, the Direct, G&S and Indirect methods showed a positive correlation with the PLTO method. The Bland-Altman test demonstrated that the G&S method presented a high agreement with the PLTO method. Conclusion: The G&S method is inexpensive, easy to perform, and has demonstrated statistical reliability concerning the automated methodology, and is useful for confirming low platelet counts after a suspected error by the automated equipment or when this device is not available for use.
Highlights
The history of muscle biopsy dates back to 1860, when Duchenne first performed a biopsy on a patient with symptoms of myopathy[1]
The twenty-first century has brought in a new spectacular progress in the utility of muscle biopsy with the commencement of molecular methods
The molecular era was made possible by the development of molecular biology and its application to muscle diseases
Summary
The history of muscle biopsy dates back to 1860, when Duchenne first performed a biopsy on a patient with symptoms of myopathy[1]. The introduction of enzyme histochemical methods by Victor Dubowitz, in 1970, revolutionized the role of muscle biopsy in the diagnosis of various primary and secondary muscle diseases[2]. The adaptation of histo- and cytochemical techniques to the study of muscle biopsies improved diagnostic accuracy and enabled the identification of new changes and structures[3, 4].
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