Validated UV-Spectrophotometric Methods for Determination of Gemifloxacin Mesylate in Pharmaceutical Tablet Dosage Forms

P Pingle Sunita,R Rote Ambadas

doi:10.1155/2010/346847

Abstract

Two simple, economic and accurate UV spectrophotometric methods have been developed for determination of gemifloxacin mesylate in pharmaceutical tablet formulation. The first UV-spectrophotometric method depends upon the measurement of absorption at the wavelength 263.8 nm. In second area under curve method the wavelength range for detection was selected from 268.5-258.5 nm. Beer’s law was obeyed in the range of 2 to 12 μgmL-1for both the methods. The proposed methods was validated statistically and applied successfully to determination of gemifloxacin mesylate in pharmaceutical formulation.

Highlights

Gemifloxacin (R, S)-7(3-aminomethyl-4-syn-methoxyimino-1-pyrrolidinyl)-1-cyclopropyl-6fluro-1, 4 dihydro-4-oxo-1, 2 naphthyridine-3-carboxylic acid is a new fluroquinolone antibacterial compound with enhanced affinity for bacterial topoisomerase IV and has been developed for the treatment of respiratory and urinary tract infection
Ten mL of stock solution was further diluted to 100 mL with methanol to get a working standard solution of concentration 100 μg mL-1.This solution used as standard working solution
From the working stock solutions appropriate dilutions of gemifloxacin mesylate were made with distilled water

Summary

Introduction

Gemifloxacin (R, S)-7(3-aminomethyl-4-syn-methoxyimino-1-pyrrolidinyl)-1-cyclopropyl-6fluro-1, 4 dihydro-4-oxo-1, 2 naphthyridine-3-carboxylic acid is a new fluroquinolone antibacterial compound with enhanced affinity for bacterial topoisomerase IV and has been developed for the treatment of respiratory and urinary tract infection. Standard stock solution of gemifloxacin mesylate was prepared by dissolving 25 mg of gemifloxacin mesylate in 25 mL of methanol to get concentration of 1.0 mg mL-1 in volumetric flask. A 1 mL aliquot of sample stock solution was transferred to 10 mL standard volumetric flask and volume was made up to mark with distilled water.

Results

Conclusion