Abstract
Purpose : To evaluate the total phenolic content and antioxidant potential of the methanol extracts of aerial parts and roots of Thymus sipyleus Boiss and also to determine some phenolic compounds using a newly developed and validated reversed phase high performance liquid chromatography (RP-HPLC) method. Methods : The total phenolic concentration of the extracts were determined using Folin Ciocalteu method. The antioxidative potential of the samples was evaluated using DPPH and ABTS assays. Phenolics responsible for the antioxidant activity of the plant were quantified by a newly developed and validated RP-HPLC method for the first time. Results : The total phenolic concentration of the aerial parts and roots were 215.1 ± 2.24 and 152.25 ± 2.31 mg GAE/g extracts, respectively. The extract obtained from the aerial parts reduced DPPH with a half-maximal inhibition concentration (IC 50 ) of 0.703 ± 0.027 mg/mL, while the IC 50 obtained from ABTS assay was 0.869 ± 0.066 mg/mL. IC 50 values of Trolox used as standard for DPPH and ABTS radical scavenging assays were 0.0430 ± 0.0001 and 0.0420 ± 0.0001 mg/mL, respectively. The results revealed that T. sipyleus contains remarkable amounts of rosmarinic acid in the aerial parts (0.8887 ± 0.0016 g/100g dw) and in the roots (0.3454 ± 0.0006 g/100g dw) which is believed to be mostly responsible for the observed activity. Conclusion : T. sipyleus is a major potential antioxidant source for the food, pharmaceutical and cosmetic industry due to its high contents of rosmarinic acid and total phenolics. Keywords : Thymus sipyleus, Rosmarinic acid, Chlorogenic acid, Caffeic acid, Apigenin, Antioxidant
Highlights
Plants are valuable sources of natural compounds with different chemical and biological properties
This paper reports the qualitative and quantitative analysis of phenolics in different parts of Thymus sipyleus for the first time, together with antioxidant activity and total phenolic content
For the total phenolic content and antioxidant activity tests, 5 g of air dried and milled aerial parts and roots of the plant were extracted with methanol (≥ 99.9 %, 100 mL) using a magnetic stirrer for 1 h (50 °C, 250 rpm)
Summary
Plants are valuable sources of natural compounds with different chemical and biological properties. Plant secondary metabolites and extracts are of great interest during the last few decades due to their various pharmacological activities. The wide usage of plants as food, food additives, health promoting agents and supplements has undoubtedly increased the number of studies which aim to set light to the chemical composition and biological activity of these sources [1,2,3]. Oxidative stress which can be defined as the imbalance between oxidants and antioxidants, elicit several pathophysiological disorders such as cancer, aging and other diseases. It’s clear that fighting with these parameters plays an important role in the prevention of chronic diseases. It is very important to consider plants first in order to eliminate the risk of such
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