Abstract
This paper describes a new, simple, precise, and accurate HPTLC method for simultaneous estimation of Atenolol and Aspirin as the bulk drug and in tablet dosage forms. Chromatographic separation of the drugs was performed on aluminum plates precoated with silica gel 60 F254 as the stationary phase and the solvent system consisted of n-butanol : water : acetic acid (8 : 2 : 0.2 v/v/v). Densitometric evaluation of the separated zones was performed at 235 nm. The two drugs were satisfactorily resolved with values and for Atenolol and Aspirin, respectively. The accuracy and reliability of the method was assessed by evaluation of linearity (100–600 ng/spot for Atenolol and Aspirin), precision (intraday % RSD was 0.48–1.03 and interday % RSD was 0.68–1.14 for Atenolol, and intraday % RSD was 0.61–1.03 and interday % RSD was 0.69–1.04 for Aspirin), accuracy ( for Atenolol and for Aspirin), and specificity in accordance with ICH guidelines.
Highlights
Atenolol is chemically (RS)-2-{4-[2-hydroxy-3-(propan-2ylamino)propoxy]phenyl} acetamide (Figure 1)
Literature review reveals that methods have been reported for analysis of Atenolol and Aspirin by UV spectrophotometry [3, 4], method development and validation either alone or in combination with other drugs [5,6,7,8,9,10], and stability indicating analytical method by HPTLC [11]
To date there have been no published reports on simultaneous quantitation of Atenolol and Aspirin by HPTLC in bulk drug and in tablet dosage form. This present study reports for the first time the simultaneous quantitation of Atenolol and Aspirin by HPTLC in bulk drug and in tablet dosage form
Summary
Atenolol is chemically (RS)-2-{4-[2-hydroxy-3-(propan-2ylamino)propoxy]phenyl} acetamide (Figure 1). Atenolol is a beta-adrenergic blocking agent that blocks the effects of adrenergic drugs. Aspirin chemically is 2-acetoxybenzoic acid (Figure 2). Aspirin’s ability to suppress the production of prostaglandins and thromboxanes is due to its irreversible inactivation of the cyclooxygenase (COX) enzyme. Literature review reveals that methods have been reported for analysis of Atenolol and Aspirin by UV spectrophotometry [3, 4], method development and validation either alone or in combination with other drugs [5,6,7,8,9,10], and stability indicating analytical method by HPTLC [11]. To date there have been no published reports on simultaneous quantitation of Atenolol and Aspirin by HPTLC in bulk drug and in tablet dosage form.
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