Abstract
BackgroundIn the present study, an HPTLC (high-performance thin-layer chromatography) method was developed for the quantitative determination and validation of the curcumin in the methanolic fraction of Curcuma longa L. For achieving good separation of curcumin, the mobile phase of chloroform:methanol (97:3) was used. The densitometric analysis of curcumin was performed at 420 nm in reflection/absorption mode.ResultsLinearity of the method was obtained in the range of 100‒600 ng per spot. During analysis, the methanolic fraction of the C. longa showed the presence of a quantifiable amount of curcumin. The content of curcumin was found to be 1.5% (per dry weight).ConclusionsThe method is specific, simple, precise, and accurate. The obtained data can have used for the routine analysis of the reported biomarkers in crude drugs and extracts. The quantification and the method validation of curcumin have not yet been reported in C. longa which can be utilized for the proper standardization of the plant.
Highlights
In the present study, an High-Performance Thin-Layer Chromatography (HPTLC) method was developed for the quantitative determination and validation of the curcumin in the methanolic fraction of Curcuma longa L
High‐performance thin‐layer chromatographic method optimization To provide an accurate, precise, and reproducible method for the determination of curcumin, HPTLC conditions were optimized in terms of selection of mobile phase, absorption maxima, and slit dimensions
High‐performance thin‐layer chromatographic method validation HPTLC profile (Fig. 4) with chromatogram was obtained for the standard compound, and the plant extract and targeted compound from the targeted plant
Summary
An HPTLC (high-performance thin-layer chromatography) method was developed for the quantitative determination and validation of the curcumin in the methanolic fraction of Curcuma longa L. It is a medicinally important plant commercially known as Haldi, Turmeric, or Indian saffron. It is a natural polyphenol procured from the rhizomes of C. longa [3]. Curcumin is included in various herbal remedies to treat skin inflammation and its infection. It is therapeutically used for the treatment of amenorrhea, dislocation of joints, diarrhea, diabetes, liver disorder, bronchitis, ringworm infection, toothache, anemia, and weakness of eyesight [1, 4, 5]
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