Abstract
It has been shown by chromatography that aglycone, glucoside, acetylglucosideand malonylglucoside isoflavone extracts prepared from soybean cake showed betterantioxidant activities than isoflavone standards. Consequently, the aim of this study was toevaluate the protective effects of these isoflavone extracts against ultraviolet B (UVB)-induced keratinocyte damage. Our results demonstrated that these soybean cake isoflavoneextracts could inhibit UVB-induced keratinocyte death. Moreover, they could inhibit UVB-induced intracellular release of hydrogen peroxide (H2O2) Furthermore, these isoflavoneextracts differentially inhibited UVB-induced MAPK phosphorylation. The ERK1/2 andp38 phosphorylation was not inhibited by all tested isoflavone extracts, whereas JNKphosphorylation was inhibited by group I to group III isoflavone extracts. Since theseisoflavone extracts are relative stable and easily obtained than the isoflavone standards, wesuggest that soybean cake may be a useful potential source for developing effective skincare agents in against photoaging.
Highlights
Diets rich in soybean products are associated with reduced incidence of cardiovascular disease, osteoporosis and certain human cancers [1,2,3]
To determine protective effects of isoflavone extracts obtained from soybeans on ultraviolet B (UVB)-irradiated keratinocytes, cell viability was determined by the MTT assay
These results indicate that aglycone, glucoside, acetylglucoside and malonylglucoside isoflavone extracts are able to prevent UVB-induced human skin damage
Summary
Diets rich in soybean products are associated with reduced incidence of cardiovascular disease, osteoporosis and certain human cancers [1,2,3]. Animal studies have shown that soy diets block oxidative degradation of lipoproteins, prevent atherosclerosis of blood vessels and inhibit radiationand carcinogen-induced tumors of various tissues. Soybeans contain ingredients with potential biological effects and isoflavone is one of the most extensively studied ingredients. Twelve isoflavones are found in soybean and are present in four chemical forms: malonylglucosides (malonyldaidzin, malonylgenistin, and malonylglycitin), acetylglucosides (acetyldaidzin, acetylgenistin, and acetylglycitin), glucosides (daidzin, genistin, and glycitin) and aglycones (daidzein, genistein, and glycitein). In vitro and in vivo studies have shown that isoflavone aglycones such as genistein inhibit ultraviolet light-induced damage [6,7,8]. It has been shown that isoflavone glycosides such as genistin, daizin and glycitin have biological activities on melanoma cells and myoblasts [7,9]. It is possible that aglycones and glycosides can show some physiological relevance
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