UV Spectrophotometry with Chemometric methods for Concurrent Assays of Antihypertensive Components in Tablets

Abstract

Chemometrics is the precise and practice of analyzing data through various numerical techniques in order to develop useful information. Generally, it is better to measure many nonselective signals and then combine them in a multivariate model. Nowadays, the combinations of amlodipine, valsartan, and hydrochlorothiazide are effective antihypertensive drugs. The combination of active ingredients will be evaluated for its component content and validated using the chemometric spectrophotometric method paired with the multivariate partial least squares system. For modeling calibration and chemometric validation, a double divisor system was employed. An ultraviolet spectrophotometric measurement was performed by measuring the absorbance spectrum at a wavelength of 230-300nm of AML, 220-290nm of valsartan, and 230-300nm of hydrochlorothiazide at 2nm intervals. Partial Least Squares were used to calculate levels of AML is (99.59±0.28)%; VAL is (101.99± 0.44)% and HCT is (103.60±0.34)% and cross-validated using R2, PRESS, and RMSCEV. Concluded that the determination of amlodipine, valsartan, and hydrochlorothiazide mixtures by the partial least squares multivariate chemometric method met the requirements for tablet dosage forms, according to USP 43-NF38.