Abstract
AbstractA simple and rapid method for determining the degree of N‐acetylation (DA) of chitosan by ultraviolet spectrophotometry using double beam equipment is proposed. D‐Glucosamine (GlcN) hydrochloride and N‐acetylglucosamine (GlcNAc) were used as model substances, and chitosan solutions in different solvents were studied. The best results were obtained from 0.1 M HCl solutions, with high reproducibility and in good agreement with those given by conductometric titration, FT‐IR, 1H NMR and elemental analysis.Correlation between DA obtained by UV and DA obtained by conductometric titration.magnified imageCorrelation between DA obtained by UV and DA obtained by conductometric titration.
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